README for Figure S1d.csv 
*** This file contains the raw data obtained on DNA, DNA-MvaT and DNA-MvaT-gp4 complexes using bridging assay experiments represented in Figure S1d of 

Article: Novel anti-repression mechanism of H-NS proteins by a phage protein
Authors: Ben Bdira, Erkelens, Qin, Volkov, Lippas, Bowring, Boyle, Ubbink, Dove, Dame 
Journal: Nucleic Acids Research 

Corresponding authors: fredjbdira@gmail.com and rtdame@chem.leidenuniv.nl 

Legend Figure S1: (a) Electrophoretic mobility shift assay (EMSA) of a 200bp DNA substrate at different
concentrations of MvaT wild type (upper panel), MvaT F36D/M44D (middle panel) and gp4 (lower
panel). (b)&(c) Schematic representations of the TPM and bridging assays, respectively, of the
experimental data shown in figure (1f,g). (d) Time dependent bridging assay of DNA-MvaT-DNA
complexes. The blue curve represents the kinetics of MvaT-DNA bridge formation without adding gp4.
The red and pink curve represent the kinetics of MvaT-DNA bridge formation with gp4 introduced at
180 s and 1200 s, respectively. The grey curve represents the introduction of gp4 at the beginning of
the assay (t = 0 s). Error bars represent the standard deviation from at least two independent
measurements. The assays were performed in the presence of 2.4 M MvaT and 2.4 M gp4 (1: molar
ratio). The orange point represents a control measurement without proteins at 1200s of incubation. (e)
Dynamic light scattering profiles of 100 M of MvaT wild type in the absence (left panel) and presence
of 500 M gp4 (right panel) in 20mM Tris-HCl, pH 8, 300 mM KCl. Note the increase in particle size
of MvaT upon complex formation with gp4. (f) Effect of the gp4 C-terminal helix (residues 30-46) on
MvaT DNA bridging activity.

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure S1d. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of MvaT added to sample in micromolar
Column E: Incubation time in seconds
Column F: Amount of gp4 added to sample in micromolar after incubation time in column E
Column G: Incubation time in seconds after addition of gp4 in column F
Column H: Counts per minutes 
Column I: DNA recovery (%)

Columns B until G indicate the contents and incubation times of the sample. Column H indicates the radioactivity of the sample. Column I indicates the DNA recovery, which is calculated by subtracting the value in column H of the control (sample minus bait DNA) from the sample itself. This value is then divided by the value in column H of sample 1 and multiplied by 100%. 









